Kendrick Laboratories (United States)
Kendrick Laboratories (United States) is a company in Madison, United States.
Does 2D Western blotting work as well as 1D?
“Yes, 2D works better because the proteins clumped together in a 1D band are further spread out in 2DE by isoelectric focusing. 2D WB is extremely sensitive and informative.”
View in the company profileWhat is the sensitivity of your stains?
“Every protein stains differently so it’s difficult to say exactly. Our in-house Coomassie blue stain is very sensitive, gives a fairly dark tropomyosin spot at 0.5 μg and a clearly discernible one at 100 ng. We find it’s roughly equal in sensitivity to the fluorescent stain Sypro Ruby.”
View in the company profileWhat is the maximum amount of protein and volume that can be loaded on a 2D gel?
“In general for complex samples such as cell lysates run on SF gels, we suggest a maximum load of 200 μg for Coomassie blue or Sypro ruby staining and 50 μg for silver staining.”
View in the company profileHow are pI and MW standardized?
“Molecular weight (MW) markers listed below are loaded in a well on the right side (basic side) of the 2D gel in a well in the agarose that seals the tube gel to the slab gel.”
View in the company profileDo you accept radiolabeled proteins?
“No. As of December 1, 2017 we will no longer accept radioactive samples.”
View in the company profile